Cell-free Protein Crystallization for Nanocrystal Structure Determination
S. Abe, J. Tanaka, M. Kojima, S. Kanamaru, K. Hirata, K. Yamashita, A. Kobayashi, T. Ueno
The paper develops cell-free protein crystallization (CFPC) to produce high-quality protein nanocrystals for structure determination, achieving a 2.11 Å resolution structure for CipA.
bioRxiv · Apr 15, 2022 · PreprintBiophysics