Use of Transient Transfection for cGMP Manufacturing of eOD-GT8 60mer, a Self-Assembling Nanoparticle Germline-Targeting HIV-1 Vaccine Candidate
V. K. Sharma, V. Tsvetnitsky, S. Menis, E. T. Brower, E. Sayeed, J. Ackland, A. Lombardo, T. Hassell, W. R. Schief
This paper describes the cGMP production and characterization of eOD-GT8 60mer, a self-assembling HIV-1 vaccine nanoparticle, using transient transfection in HEK293 cells and subsequent purification techniques.
bioRxiv · Sep 30, 2022 · PreprintBiochemistry