W. R. Schief

This paper introduces new mouse models expressing human HIV-1 broadly neutralizing antibody (bnAb) precursors with more physiological levels and diversity, enhancing vaccine research for bnAb elicitation.

This paper describes the cGMP production and characterization of eOD-GT8 60mer, a self-assembling HIV-1 vaccine nanoparticle, using transient transfection in HEK293 cells and subsequent purification techniques.

The paper identifies IL-2's role in promoting B cell maturation by regulating the mTOR/AKT/Blimp-1 axis through T helper cell interactions.

This paper introduces a method for efficiently isolating rare antigen-specific B cells using next-generation antigen barcoding and a computational framework, demonstrating its ability to recover thousands of mAbs, including rare HIV broadly neutralizing antibodies.

This study applies Bcl-6/Bcl-xL retroviral transduction to isolate antibodies and generate B cell clones from various B cell populations in healthy and infected individuals.

The paper identifies a broadly neutralizing epitope in SARS-related coronaviruses that is effective against variants, including Omicron, and suggests its use for universal vaccine design.

This study shows that germinal center B cells can persist and continue to mutate for 29 weeks after a single immunization, leading to long-lasting antibody responses.

The paper shows that spatially restricted protease activity in lymph node follicles preserves antigen integrity, leading to stronger GC responses when antigens are targeted to follicular dendritic cells.

DeGlyPHER is an ultrasensitive method to analyze viral spike N-glycoforms, assessing glycan occupancy and processing from high-mannose to complex forms at each N-glycosylation site.

This paper uses cryoEM and next-generation sequencing to identify monoclonal antibodies from polyclonal sera and validate their antigen-binding properties.

The paper screens naive B cells for VRC01-class broadly neutralizing antibody precursors using a germline-targeting immunogen and single-cell BCR sequencing, finding common germline variation affecting gene usage.

The paper uses cryoEM to analyze polyclonal antibody responses to HIV Env immunogens in rhesus macaques, revealing structural differences in antibody binding and immunogenicity of engineered mutations and glycan sites.

This study demonstrates that minor groove binders can protect wireframe DNA origami from endonuclease degradation, enhancing their stability in biological environments.

CoV3D provides a database of coronavirus protein structures, including spike glycoprotein complexes, for vaccine and therapeutic design.

The paper demonstrates that authentic naive human VRC01-class BCRs can be primed and recruited to germinal centers in multiple mouse models, validating germline-targeting vaccine design.

This study shows that HIV Env immunogens can be concentrated in B cell follicles using immune complexes or protein nanoparticles, enhancing their localization in lymph nodes and potentially improving immune responses.

This study uses DNA origami to investigate how antigen organization affects B-cell activation, finding that optimal spacing and valency enhance signaling.

This study presents methods to incorporate full-length HIV-1 Env into lipid assemblies for structural analysis, revealing the quaternary epitope of an MPER antibody and Env tilting during neutralization.

This study shows that high serum neutralizing antibody titers from Env protein vaccination protect rhesus macaques from Tier 2 SHIV challenge, with ~1:500 ID50 titers providing over 90% protection.

This study shows that slow delivery immunization in rhesus macaques increases HIV-neutralizing antibody titers and germinal center responses by modulating immunodominance.

This paper introduces RosettaAntibodyDesign (RAbD), a computational framework for antibody design that improves affinity by redesigning CDRs using structural and sequence-based methods.