Hybrid-DIA: Intelligent Data Acquisition for Simultaneous Targeted and Discovery Phosphoproteomics in Single Spheroids
Ana Martínez‐Val, Kyle L. Fort, Claire Koenig, Leander van der Hoeven, Giulia Franciosa, Thomas Moehring, Yasushi Ishihama, Yu‐Ju Chen, Alexander Makarov, Yue Xuan, Jesper Velgaard Olsen
The paper introduces hybrid-DIA, a method combining targeted and discovery phosphoproteomics for single spheroids, showing comparable accuracy to existing methods while profiling the global phosphoproteome.
Nature Communications · Jun 16, 2023Peer-reviewedSystems Biology