Kyle L. Fort

Full Scan enhanced Dynamic Range MS improves metabolite coverage and cancer cell-line discrimination in untargeted metabolomics

This paper introduces Full Scan enhanced Dynamic Range MS to improve metabolite detection in untargeted metabolomics, increasing feature detection and enabling better discrimination between cancer cell lines.

Orbitrap Collision Cross Section Measurements Enhance Isomer Annotations in Lipidomics

This paper introduces a method to infer lipid CCS values from LC-Orbitrap MS data, improving isomer annotation in lipidomics with high precision and accuracy.

Higher-Throughput Proteome Profiling Enabled by Parallelized Pre-Accumulation and Optimized Ion Processing in the Orbitrap Astral Zoom Mass Spectrometer

Orbitrap Astral Zoom upgrades enable 270 Hz DIA scanning, identifying 7184 protein groups at 500 samples/day and >12,000 proteins from fractionated HEK293 in 2.7 h.

Increased EThcD efficiency on the hybrid Orbitrap Excedion Pro Mass Analyzer extends the depth in identification and sequence coverage of HLA class I immunopeptidomes

This study shows that using EThcD on a new mass spectrometer improves the identification and sequence coverage of HLA class I immunopeptidomes by filling gaps in fragmentation data.

Enhancing tandem MS sensitivity and peptide identification via ion pre-accumulation in an Orbitrap mass spectrometer

This paper introduces a new scanning strategy called pre-accumulation to enhance ion utilization and sensitivity in Orbitrap mass spectrometers, improving peptide and protein identification in high-throughput proteomics.

To 200,000 m/z and beyond: native electron capture charge reduction mass spectrometry deconvolves heterogeneous signals in large biopharmaceutical analytes

This paper demonstrates the use of electron-capture charge reduction (ECCR) to deconvolve heterogeneous signals in native mass spectrometry, achieving m/z values above 200,000 for large biopharmaceutical analytes.

Hybrid-DIA: Intelligent Data Acquisition for Simultaneous Targeted and Discovery Phosphoproteomics in Single Spheroids

The paper introduces hybrid-DIA, a method combining targeted and discovery phosphoproteomics for single spheroids, showing comparable accuracy to existing methods while profiling the global phosphoproteome.

Characterisation of an Omnitrap-Orbitrap platform equipped with IRMPD, UVPD and ExD for the analysis of peptides and proteins

The paper characterizes an Omnitrap-Orbitrap mass spectrometer with multiple ion activation methods, showing its versatility in peptide and protein analysis.

Mass-selective and ice-free cryo-EM protein sample preparation via native electrospray ion-beam deposition

This paper introduces native ES-IBD for preparing high-purity, ice-free cryo-EM samples, showing structural integrity but limitations in 2D classes and 3D EM density maps.