Conformational gating of product release sets the catalytic ceiling of a bioluminescent reporter
Martin Toul, Jana Horáčková, Andrea Schenkmayerová, Joan Planas-Iglesias, Tomas Landolt, Jeerus Sucharitakul, Yves Louis Janin, Kridsadakorn Prakinee, Pimchai Chaiyen, Stavros Stavrakis, Andrew J. deMello, Kenneth Allen Johnson, Jiřı́ Damborský, Martin Marek, David Bednář, Zbyněk Prokop
This study identifies conformational dynamics as a key determinant of luciferase catalytic efficiency and light-emission stability, revealing a trade-off between oxygen affinity and enzyme longevity.
bioRxiv · Sep 16, 2025 · PreprintBiochemistry