Kenneth Allen Johnson

The paper compares Cas12f orthologs to identify mechanistic features that enhance genome editing efficiency in human cells.

The paper develops a biosensor to measure intracellular ADP-ribose dynamics and finds that ADPR levels are transient, tunable, and critical for TRPM2 activation.

This study identifies conformational dynamics as a key determinant of luciferase catalytic efficiency and light-emission stability, revealing a trade-off between oxygen affinity and enzyme longevity.

This study reports cryo-EM structures of Cas9d, revealing its DNA targeting mechanism and demonstrating a compact variant with robust nuclease activity in mammalian cells.

This study defines the kinetic mechanism and rate constants for non-LTR retrotransposition using single-turnover analysis, revealing the complete reaction sequence and second-strand synthesis.