CyanoTag: Discovery of protein function facilitated by high-throughput endogenous tagging in a photosynthetic prokaryote
A. Perrin, M. Dowson, A. Dowle, G. Calder, V. Springthorpe, G. Zhao, L. Mackinder
The paper introduces CyanoTag, a high-throughput method for endogenous fluorescent protein tagging in Synechococcus elongatus, enabling subcellular localization, protein abundance tracking, and interaction network analysis of over 330 proteins.
bioRxiv · Feb 28, 2024Microbiology