Scalable cell-free production of active T7 RNA polymerase
Ryan N. Rezvani, Rochelle Aw, Wei Chan, Krishnathreya Satish, Han Chen, Adi Lavy, Swechha Rimal, Divyesh A. Patel, Govind Rao, James R. Swartz, Matthew P. DeLisa, Erik Kvam, Ashty S. Karim, Antje Krüger, Weston Kightlinger, Michael C. Jewett
This paper demonstrates scalable cell-free production of active T7 RNA polymerase with high purity and activity, suitable for decentralized biotherapeutic manufacturing.
Biotechnology and Bioengineering · Apr 29, 2025Peer-reviewedSynthetic Biology