Han Chen

Coordinated circulating and tissue-based T cell responses precede xenograft rejection

This study tracks T cell clonotypes across tissues in a pig-to-human kidney xenotransplantation model, showing coordinated immune responses preceding xenograft rejection.

Scalable cell-free production of active T7 RNA polymerase

This paper demonstrates scalable cell-free production of active T7 RNA polymerase with high purity and activity, suitable for decentralized biotherapeutic manufacturing.

AnnoTree: visualization and exploration of a functionally annotated microbial tree of life

AnnoTree visualizes and explores functional annotations across 24,000 bacterial genomes, revealing widespread phylogenetic patchiness in gene families.

The regulator-executor-phenotype architecture shaped by natural selection

The paper investigates how natural selection shapes the regulator-executor-phenotype architecture in yeast by analyzing morphological traits and their genetic control.

The non-essentiality of essential genes suggests a loss-of-function therapeutic strategy for loss-of-function human diseases

The paper identifies type II essential genes, which can become non-essential through secondary loss-of-function mutations, suggesting a therapeutic strategy for human diseases like ADSL deficiency.

Principles of studying a cell - a non-boastful paper for all molecular biologists

The paper analyzes 500 yeast traits to show observational approaches work best for traits under strong selection, while genetic approaches succeed when coordinating genes are perturbed.

Natural selection defines the cellular complexity

The paper identifies expression informative genes (EIGs) correlated with fitness traits in yeast, showing natural selection shapes simple functional architectures for fitness-coupled traits, while complex epistasis governs fitness-uncoupled traits.