Wide field-of-view, twin-region two-photon imaging across extended cortical networks
Jeffrey N. StirmaniD, Ikuko T. SmithiD, Michael W. Kudenov, Spencer L. SmithiD
Abstract
We demonstrate a two-photon imaging system with corrected optics including a custom objective that provides cellular resolution across a 3.5 mm field of view (9.6 mm2). Temporally multiplexed excitation pathways can be independently repositioned in XY and Z to simultaneously image regions within the expanded field of view. We used this new imaging system to measure activity correlations between neurons in different cortical areas in awake mice.
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