bioRxivGenomicsPreprint
Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping
Yang LiuiD, Marcello DiStasioiD, Graham SuiD, Hiromitsu AsashimaiD, Archibald EnninfuliD, Xiaoyu QiniD, Yanxiang DengiD, Pino BordignoniD, Marco CassanoiD, Mary M. TomaykoiD, Mina L. XuiD, Stephanie HaleneiD, Joseph E. CraftiD, David A. HafleriD, Rong FaniD
Abstract
We present spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping, which was firstly demonstrated for profiling 189 proteins and transcriptome in multiple mouse tissue types. It was then applied to human tissues to measure 273 proteins and transcriptome that revealed spatially distinct germinal center reaction in tonsil and early immune activation in skin at the COVID-19 mRNA vaccine injection site. Spatial-CITE-seq may find a range of applications in biomedical research.
- organism: mouse
- organism: human
- assay: CITE-seq
- assay: bulk RNA-seq
- research focus: cell type annotation
- research focus: spatial domain detection
- research focus: cell-cell communication
- approach: experimental assay
- Citations
- 21
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