Tuning the transglycosylation reaction of a GH11 xylanase by a delicate enhancement of its thumb flexibility
K. Marneth, H. van den Elst, A. Cramer-Blok, J. D. C. Codee, H. S. Overkleeft, H. M. F. G. Aerts, M. Ubbink, F. Ben Bdira
This study modifies a GH11 xylanase to enhance transglycosylation by increasing thumb flexibility through a point mutation, altering its enzymatic balance and dynamics.
bioRxiv · Dec 19, 2020Biochemistry