Limit of detection of Salmonella ser. Enteritidis using culture-based versus culture-independent diagnostic approaches
L. M. Bradford, L. Yao, C. Anastasiadis, A. L. Cooper, B. Blais, A. E. Deckert, R. J. Reid-Smith, C. H.-F. Lau, M. S. Diarra, C. Carrillo, A. Wong
This study compares the limit of detection of Salmonella ser. Enteritidis using culture-based and culture-independent methods, finding comparable LOD50 for qPCR and shotgun sequencing, but false positives in unspiked samples and lower LOD50 in feed than caecal contents.
bioRxiv · Feb 5, 2024 · PreprintMicrobiology