A Fluorogenic-Based Assay to Measure Chaperone-Mediated Autophagic Activity in Cells and Tissues
A. Jonnavithula, M. Tandar, M. Umar, S. N. Orton, M. C. Woodrum, S. Mookherjee, S. Boudina, J. D. Symons, R. Ghosh
The paper develops a fluorogenic assay to measure chaperone-mediated autophagy (CMA) activity in cells and tissues by using a KFERQ-AMC substrate and quantifying fluorescence release.
bioRxiv · Dec 14, 2023 · PreprintBiochemistry