Decreasing the flexibility of the TELSAM-target protein linker and omitting the cleavable fusion tag improves crystal order and diffraction limits
P. Gajjar, M. J. Pedroza-Romo, C. Litchfield, M. Callahan, N. Redd, S. D. Nawarathnage, S. Soleimani, J. Averett, E. Wilson, A. Lewis, C. Stewart, Y. J. Tseng, T. Doukov, A. Lebedev, J. D. Moody
This study shows that rigidifying the TELSAM-target protein linker and removing the SUMO tag improves crystal diffraction resolution.
bioRxiv · May 12, 2023 · PreprintBiochemistry