T. Doukov

Fusion crystallization reveals the behavior of both the 1TEL crystallization chaperone and the TNK1 UBA domain

The paper describes the use of TELSAM fusion crystallization to determine the structure of the TNK1 UBA domain and its interaction with polyubiquitin.

Decreasing the flexibility of the TELSAM-target protein linker and omitting the cleavable fusion tag improves crystal order and diffraction limits

This study shows that rigidifying the TELSAM-target protein linker and removing the SUMO tag improves crystal diffraction resolution.

Refinement of Multiconformer Ensemble Models from Multi-temperature X-ray Diffraction Data

This study refines multiconformer ensemble models from multi-temperature X-ray diffraction data of Proteinase K, revealing temperature-driven conformational changes and their functional implications.

High-throughput enzymology reveals mutations throughout a phosphatase that decouple catalysis and transition state analog affinity

This study uses HT-MEK to analyze 1,004 PafA mutants, showing that distal mutations affect catalysis more than TSA binding, suggesting conformational changes influence enzyme function.

Ensemble-function relationships to evaluate catalysis in the ketosteroid isomerase oxyanion hole

The paper evaluates catalytic effects in ketosteroid isomerase by analyzing ensemble conformations and their functional implications.

Cooperative Allostery and Structural Dynamics of Streptavidin at Cryogenic- and Ambient-temperature

The paper presents two apo-streptavidin structures at different temperatures and reveals cooperative allostery through water-mediated interactions.

TELSAM polymers accelerate crystallization of fused target proteins by stabilizing minimal crystal contacts and in the absence of direct inter-TELSAM contacts

This study shows that TELSAM polymers enhance protein crystallization by stabilizing minimal crystal contacts and enabling rapid crystallization without direct inter-TELSAM contacts.

Damaged goods? Evaluating the impact of X-ray damage on conformational heterogeneity in room temperature and cryo-cooled protein crystals

This study evaluates how X-ray damage affects conformational heterogeneity in protein crystals at room temperature and cryo-cooled conditions, finding that damage effects can be minimized or eliminated.

Assessment of enzyme active site positioning and tests of catalytic mechanisms through X-ray-derived conformational ensembles

This study uses X-ray crystallography to analyze enzyme active site positioning and motions, finding limited conformational changes and challenging existing catalytic mechanisms.

A Robust Method for Collecting X-ray Diffraction Data from Protein Crystals across Physiological Temperatures

This paper introduces a method for collecting high-resolution X-ray diffraction data from protein crystals at physiological temperatures, achieving high-quality data in under 5 seconds.

Structural adaptation of oxygen tolerance in 4-hydroxybutyrl-CoA dehydratase, a key enzyme of archaeal carbon fixation

The paper investigates how 4HBD, a key enzyme in archaeal carbon fixation, adapted structurally to become oxygen-tolerant, revealing evolutionary changes that enabled aerobic metabolism.

Structure of human cytomegalovirus UL144, an HVEM orthologue, bound to the B and T cell Lymphocyte Attenuator

This paper reports the crystal structure of the HCMV UL144/BTLA complex, revealing molecular mechanisms of selective BTLA binding and informing the design of BTLA agonists.

Crystal structure of m4-1BB/4-1BBL complex reveals an unusual dimeric ligand that undergoes structural changes upon receptor binding.

This paper reports the crystal structure of mouse 4-1BBL and its complex with 4-1BB, revealing unique dimerization and structural changes upon binding that explain species-specific interactions.