Resolving chaperone-assisted protein folding on the ribosome at the peptide level
T. E. Wales, A. Pajak, A. Roeselova, S. Shivakumaraswamy, S. Howell, F.-U. Hartl, J. R. Engen, D. Balchin
This study uses HDX-MS to map the cotranslational folding pathway of E. coli dihydrofolate reductase, revealing structured intermediates and chaperone interactions on the ribosome.
bioRxiv · Sep 23, 2022Biochemistry