An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein
P. S. Dewari, B. Southgate, K. Mccarten, G. Monogarov, E. O'Duibhir, N. Quinn, A. Tyrer, C. Plumb, C. Blin, R. Finch, R. B. Bressan, G. Morrison, A. M. Jacobi, M. A. Behlke, A. von Kriegsheim, , S. M. Pollard
The paper introduces an efficient, selection-free, and scalable CRISPR/Cas9-based pipeline for epitope tagging in mammalian stem cells, achieving knock-in efficiencies of 5-30%.
bioRxiv · Jan 29, 2018Cell Biology