A versatile system to introduce clusters of genomic double-strand breaks in large cell populations
Thorsten Kolb, Umar Khalid, Milena Simovic-Lorenz, Manasi Ratnaparkhe, John Wong, Anna Sophia Jauch, Peter Schmezer, Agata Rode, Shulamit Sebban, Daniel Haag, Michaela Hergt, Frauke Devens, Yosef Buganim, Marc Zapatka, Peter Lichter, Aurélie Ernst
This paper introduces a method to generate localized DNA double-strand breaks in cell populations using UV-irradiation with photomasks, enabling the study of genome rearrangements and DNA damage response.
Genes Chromosomes and Cancer · Jul 31, 2020Peer-reviewedGenomics