Strategies based on sum of peak intensities may better reflect protein expression than peak area integration in iTRAQ protein expression measurement by LC-MS/MS using a TripleTOF 5600+ platform
B. Burat, J. Gonzalez, F.-L. Sauvage, H. Aouad, H. Arnion, E. Pinault, P. Marquet, M. Essig
This study compares two iTRAQ quantitation methods, finding that sum of peak intensities better correlates with Western blot results than peak area ratios.
bioRxiv · Mar 13, 2019Cell Biology