Non-Viral Engineering of CAR-NK and CAR-T cells using the Tc Buster Transposon System™
E. J. Pomeroy, W. S. Lahr, J. W. Chang, J. B. Krueger, B. J. Wick, N. J. Slipek, J. G. Skeate, B. R. Webber, B. S. Moriarity
This paper demonstrates a non-viral method using the Tc Buster transposon system to deliver CAR constructs to primary human NK and T cells, achieving efficient gene expression and functional activity.
bioRxiv · Aug 2, 2021 · PreprintBioengineering