W. S. Lahr

This paper describes a non-viral method for efficiently integrating large DNA into primary human T cells using CRISPR-Cas9 and homology-mediated end joining, achieving high functionality and anti-tumor activity.

This paper demonstrates a non-viral method using the Tc Buster transposon system to deliver CAR constructs to primary human NK and T cells, achieving efficient gene expression and functional activity.

This study shows that CISH knockout in TIL enhances neoantigen reactivity and synergizes with PD1 blockade to improve tumor regression.

This study engineers T cells to improve 3D migration through tumor microenvironments by manipulating microtubule stability and contractility, showing enhanced migration in 3D matrices and tumors.

The paper compares CBE and ABE base editors for splice-site disruption in T-cells, finding CBEs more reliable and splice-donor targeting most effective.

This paper develops a CRISPR/Cas9-based method to edit human NK cells, enhancing their anti-cancer activity by knocking out ADAM17 and PD1.

This paper develops a CRISPR/Cas9 gRNA array system to enable efficient multiplex genome engineering by delivering up to 10 gRNAs as a single transcript.