A nanobody-based strategy for rapid and scalable purification of native human protein complexes
T. A. Stevens, G. P. Tomaleri, M. Hazu, S. Wei, V. N. Nguyen, C. DeKalb, R. M. Voorhees, T. Pleiner
This paper introduces a method for rapidly purifying native human protein complexes using nanobodies and lentiviral transduction, achieving high purity and yield in ~8 days.
bioRxiv · Mar 9, 2023Biochemistry