Absolute Quantification of Microbiota in Shotgun Sequencing Using Host Cells or Spike-Ins
A. Wallace, H. Ling, S. Gatenby, S. Pruden, C. Neeley, C. Harland, C. Couldrey
This paper introduces methods to calculate absolute microbial abundances in shotgun sequencing using spike-ins or host cells, showing consistent results with qPCR and manufacturer values.
bioRxiv · Aug 23, 2023 · PreprintMicrobiology