Genetic evidence reveals the indispensable role of the rseC gene for autotrophy and the importance of a functional electron balance for nitrate reduction in Clostridium ljungdahlii
C.-M. Klask, B. Jäger, L. T. Angenent, B. Molitor
This paper uses CRISPR-Cas12a to delete genes in Clostridium ljungdahlii, showing that rseC is essential for autotrophy and that nitrate reduction is linked to energy metabolism.
bioRxiv · Aug 3, 2021Microbiology