B. Molitor

The deletion of aldehyde:ferredoxin oxidoreductase-encoding genes in Clostridium ljungdahlii results in changes in the product spectrum with various carbon sources

This study deletes AOR genes in C. ljungdahlii and shows changes in ethanol and acetic acid production with different carbon sources.

Proton-pump inhibitors increase C. difficile infection risk by altering pH rather than by affecting the gut microbiome based on a bioreactor model

An integrated systems-biology platform for power-to-gas technology

This paper develops a systems-biology platform for Methanothermobacter spp. to optimize power-to-gas technology by analyzing genome-scale metabolic models and growth performance in bioreactors.

The targeted deletion of genes responsible for expression of the Mth60 fimbriae leads to loss of cell-cell connections in M. thermautotrophicus ΔH

This study deletes genes encoding Mth60 fimbriae in M. thermautotrophicus ΔH, showing a loss of cell-cell connections and altered biofilm formation.

Genetic evidence reveals the indispensable role of the rseC gene for autotrophy and the importance of a functional electron balance for nitrate reduction in Clostridium ljungdahlii

This paper uses CRISPR-Cas12a to delete genes in Clostridium ljungdahlii, showing that rseC is essential for autotrophy and that nitrate reduction is linked to energy metabolism.

A shuttle-vector system allows heterologous gene expression in the thermophilic methanogen Methanothermobacter thermautotrophicus ΔH

This paper develops a shuttle-vector system for genetic modification of Methanothermobacter thermautotrophicus, enabling heterologous gene expression and growth on alternative substrates.

An open-source multiple-bioreactor system for replicable gas-fermentation experiments: Nitrate feed results in stochastic inhibition events, but improves ethanol production of Clostridium ljungdahlii with CO2 and H2