genetic perturbation / CRISPR

The paper shows that glutamate and neuropeptide signaling in C. elegans males convert brief mate contact into a persistent search behavior.

This study shows non-coding RNA disrupts enhancer-promoter interactions when increased, and stabilizes them when decreased, independent of transcription initiation.

This study identifies TRIM11 as a regulator of EWSR1::ATF1 stability in clear cell sarcoma, showing its depletion leads to metabolic rewiring and cell death, with PCK1 as a potential biomarker.

This study uses gene-resolved metagenomics to analyze nitrogen transformation potential in a landfill leachate SBR microbiome, revealing gene distribution across taxa and potential nitrogen retention pathways.

This study shows KAT6 and KAT7 maintain mitotic chromatin acetylation and composition, supporting postmitotic transcriptional memory and developmental competence in stem cells.

This paper identifies a TMEM175 mutation causing short QT syndrome and sudden cardiac death by disrupting lysosomal-mitochondrial interactions and impairing mitochondrial function.

The paper uses in vivo single-cell CRISPR screens to study how tumor suppressor loss and genetic perturbations affect glioma states and immune recruitment in mice.

The paper identifies essential C1-fixing cluster genes in Clostridium autoethanogenum using gene deletions and functional analysis, revealing their roles in autotrophy and metabolism.

The paper identifies SEC16A/MAG5 as essential for ABA receptor homeostasis by mediating ER export and trafficking, impacting ABA signaling without affecting ABA levels.

This study identifies fss genes that regulate stalked cell morphogenesis and cell cycle progression independently of the flagellum in Caulobacter crescentus.

This study identifies SAMP1 as a protector of alveolar type 2 cells against DNA damage-induced senescence and shows its role in limiting pulmonary fibrosis in a mouse model.

This study demonstrates the transferability of homoserine lactone sensors between Bacillales bacteria, enabling programmable interphyla communication between Gram-negative and Gram-positive species.

This study identifies three distinct pathways maintaining kinetochore integrity during meiosis in budding yeast, with distinct temporal requirements and interactions involving Ctf19cCCAN, Mif2CENP-C, and Cse4CENP-A-Okp1CENP-Q-Ame1CENP-U.

The paper identifies a druggable CSNK2A1-MeCP2 regulatory axis linking Okur-Chung neurodevelopmental syndrome with MECP2-related disorders through CRISPR screens and mouse models.

The paper investigates plumage diversity in Capuchino Seedeaters by analyzing gene expression and variants, finding that pigmentation differences arise from regulatory changes rather than differential gene expression.

The paper identifies oleate depletion as a metabolic signal sensed by C. elegans to activate innate immunity against bacterial infection.

This study shows that the lncRNA FOSIL regulates the timing and resolution of FOS induction by modulating chromatin structure and nuclear speckle dynamics.

The paper maps gene regulators of endothelial cell differentiation using CRISPR and single-cell RNA-seq, identifying Hippo mechanosignaling as critical for endothelial identity.

This study identifies ferroptosis in embryonic lung and kidney progenitor cells, showing its role in organ morphogenesis through genetic manipulation of Gpx4.

The paper shows RNA polymerase backtracking promotes type III-A CRISPR DNA targeting by inhibiting GreA anti-backtracking factors, which suppress Cas10-Csm activity.

The paper identifies a new Serratia taxon in mites with genomic signs of host adaptation but phylogenetically distinct from S. symbiotica.

This paper creates a CRISPR-Cas9 CYP11B1-knockout human epidermal model to study the effects of blocking cutaneous glucocorticoid synthesis.

The paper shows serotonin signaling spatially controls nutrient-responsive midgut turnover in Drosophila by regulating enteroblast maturation and morphological changes through the 5HT7 receptor.

This study identifies an mTORC1-PWP1-Hedgehog circuit that links nutrient metabolism to intestinal stem cell differentiation in Drosophila, showing its role in aging and tissue renewal.

This study shows Apelin signaling regulates neuroendocrine homeostasis in zebrafish via Atoh8-dependent transcriptional control, affecting development, fertility, and aging.

The paper shows PROM1 activation in liver cancer cells shifts their state along a preexisting differentiation axis, suggesting CD133's role extends beyond being a stem-like marker.

This study identifies DPYD's role in sleep regulation through glial lipid metabolism and myelination across species, linking genetic variants to oligodendrocyte-specific control.

This study uses AAV-delivered LplA to rescue lethal mitochondrial lipoylation defects in Lipt1-deficient mice, improving survival, motor function, and cardiac health.

This paper introduces a method to rapidly genotype zebrafish mutants using visual inspection by inserting fluorescent markers into target genes, enabling non-invasive identification of genotypes.

This study shows that proteolytic feedback regulates quorum sensing in Pseudomonas aeruginosa by controlling RhlR stability, affecting virulence and biofilm formation.