Covalent Leader Peptide Probes Enable Proteome-Level Mapping of RiPP Enzymes beyond Biosynthetic Gene Clusters
Lan Wang, Boning Wang, Ying Wang, Yundan Zheng, Yinzheng Xia, Xiangqian Xie, Ciji Wang, Tian Tian, Jing Zhao, Huijie Pan, Huan Wang
This study develops covalent leader peptide probes to identify RiPP enzymes outside biosynthetic gene clusters, revealing cross-cluster enzymatic interactions.
bioRxiv · Jan 18, 2026 · PreprintBiochemistry