Visualizing structure-mediated interactions in supercoiled DNA molecules
S. Scott, Z. M. Xu, F. Kouzine, D. J. Berard, C. Shaheen, B. Gravel, L. Saunders, A. Hofkirchner, C. Leroux, J. Laurin, D. Levens, C. J. Benham, S. R. Leslie
The paper visualizes how DNA supercoiling and temperature affect probe binding to an unwinding site using CLiC imaging, showing increased binding with higher temperature and negative supercoiling.
bioRxiv · Mar 28, 2018Biophysics