Chemical mapping exposes the importance of active site interactions in governing the temperature dependence of enzyme turnover
S. D. Winter, H. B. Jones, D. Rasadean, R. Crean, M. J. Danson, D. Pantos, G. Katona, E. Prentice, V. Arcus, M. van der kamp, C. R. Pudney
The paper maps how substrate interactions affect enzyme temperature dependence and global protein dynamics using a promiscuous enzyme.
bioRxiv · Jun 25, 2021Biochemistry