High-throughput enzymology reveals mutations throughout a phosphatase that decouple catalysis and transition state analog affinity
C. J. Markin, D. A. Mokhtari, S. Du, T. Doukov, F. Sunden, P. M. Fordyce, D. Herschlag
This study uses HT-MEK to analyze 1,004 PafA mutants, showing that distal mutations affect catalysis more than TSA binding, suggesting conformational changes influence enzyme function.
bioRxiv · Nov 7, 2022 · PreprintBiochemistry