A virion-based assay for glycoprotein thermostability reveals key determinants of filovirus entry and its inhibition
R. H. Bortz, A. C. Wong, M. G. Grodus, H. Recht, M. C. Pulanco, G. Lasso, S. J. Anthony, E. Mittler, R. K. Jangra, K. Chandran
This paper introduces an ELISA-based assay to measure the thermostability of filovirus glycoproteins, revealing how proteolytic cleavage, acid pH, and small-molecule inhibitors affect GP stability and viral entry.
bioRxiv · Feb 25, 2020Microbiology