Dark nanodiscs as a model membrane for evaluating membrane protein thermostability by differential scanning fluorimetry
J. A. Selvasingh, E. F. McDonald, J. R. Mckinney, K. V. Ledwitch, J. Meiler
This paper introduces a method using dark nanodiscs and nanoDSF to measure membrane protein thermostability, showing a biphasic unfolding pattern for DsbB with two distinct melting temperatures.
bioRxiv · May 8, 2023 · PreprintBiophysics