Short hairpin RNAs artifactually impair cell growth and suppress clustered microRNA expression.
J. Powers, E. L. da Rocha, D. Pearson, P. Missios, T. Y. de Soysa, J. Barragan, P. Cahan, G. Daley
This study shows that shRNAs impair cell growth and suppress microRNA expression in a sequence-independent manner, highlighting potential artifacts in cancer research.
bioRxiv · Dec 11, 2018 · PreprintCancer Biology