J. Gowrishankar

Pathological R-loops in bacteria from engineered expression of endogenous antisense RNAs whose synthesis is ordinarily terminated by Rho

The paper shows engineered antisense RNAs in bacteria generate R-loops that disrupt genome integrity, confirming Rho's role in preventing toxic R-loops.

The membrane-targeting-sequence motif is required for exhibition of recessive resurrection in Escherichia coli RNase E

This study identifies a membrane-targeting-sequence motif in Escherichia coli RNase E that is essential for recessive resurrection, a phenomenon where mutant subunits restore activity in hetero-tetramers.

Role for DNA double strand end-resection activity of RecBCD in control of aberrant chromosomal replication initiation in Escherichia coli

The paper shows that reduced RecBCD end-resection activity in E. coli mutants rescues DnaA loss lethality under specific DNA damage conditions, suggesting a role in preventing aberrant replication.

Essential role for an isoform of Escherichia coli translation initiation factor IF2 in repair of two-ended DNA double-strand breaks

This paper shows that IF2-1, an isoform of E. coli translation initiation factor IF2, is essential for repairing two-ended DNA double-strand breaks by facilitating RecA-mediated annealing during homologous recombination.

Modulation of RecFORQ- and RecA-mediated homologous recombination in Escherichia coli by isoforms of translation initiation factor IF2

The paper shows that IF2-1 modulates RecFORQ- and RecA-mediated homologous recombination in E. coli, suppressing synthetic lethality in uvrD-ruv and rho-ruv mutants.

Topoisomerase I Essentiality, DnaA-independent Chromosomal Replication, and Transcription-Replication Conflict in Escherichia coli

This paper shows that topA is essential for E. coli viability and identifies transcription-replication conflicts and cSDR as causes of lethality in topA mutants.