Identification of the aldolase responsible for the production of 22-hydroxy-23,24-bisnorchol-4-ene-3-one from natural sterols in Mycolicibacterium smegmatis
G. Hernandez-Fernandez, M. G. Acedos, J. L. Garcia, B. Galan
The paper identifies an aldolase gene in Mycolicibacterium smegmatis that converts a sterol intermediate into a by-product, and shows its deletion improves the yield of a desired steroid synthon.
bioRxiv · Feb 10, 2023Bioengineering