Multi-pass, single-molecule nanopore reading of long protein strands with single-amino acid sensitivity
K. Motone, D. Kontogiorgos-Heintz, J. Wee, K. Kurihara, S. Yang, G. Roote, Y. Fang, N. Cardozo, J. Nivala
This paper demonstrates a method to sequence long protein strands with single-amino-acid sensitivity using nanopore sensors and ClpX unfoldase, enabling accurate protein barcode sequencing and analysis of folded protein domains.
bioRxiv · Oct 19, 2023 · PreprintBiophysics