Site-specific genome engineering of primary human natural killer cells for programmable anti-tumor function
Vincent Allain, Allison Grace Rothrock, Pierre-Louis Bernard, William A. Nyberg, Alexis Talbot, Joseph J. Muldoon, Jing-Yi Chung, Angela To, Christopher R. Chang, Gabriella R. Kimmerly, Chang Liu, Tasha Tsao, Yasaman Mortazavi, Jin Seo, Zhongmei Li, , Justin Eyquem
This paper develops a genome engineering workflow for primary human NK cells using CRISPR/Cas9 and AAV6 to integrate and disrupt genes, enhancing anti-tumor function through targeted CAR expression.
bioRxiv · Oct 5, 2025 · PreprintImmunology