Peixin Amy Chen

This paper develops a virus-like particle-based system for CRISPR gene editing and screening in primary human myeloid cells, identifying TNFAIP3 as a key regulator of inflammatory polarization.

The paper uses CRISPR to assess the functional impact of IL2RG variants in human T cells, confirming known annotations and identifying novel loss-of-function mutations.

This paper performs in vivo CRISPR screens in human T cells to identify genetic modifiers of T cell function in solid tumors, finding P2RY8-G13 and GNAS as key regulators.

The paper improves non-viral CRISPR-Cas9 genome editing in immune cells by using truncated Cas9 target sequences and polymer-stabilized nanoparticles, enhancing efficiency and viability.