Engineering and characterisation of AAVS1 FRT cell lines for inducible and dose-dependent gene expression
Maria Portillo Malumbres, Lachlan Ellingboe, Annalisa Roberti, Oxana Nashchekina, Fernando J Sialana, Jonathon Pines, Jyoti S. Choudhary, Joerg Mansfeld, Norman E Davey
This paper develops a stable RPE1 cell line with inducible and dose-dependent transgene expression using CRISPR/Cas9 and FRT/Tet-On systems, validated through multi-omics analysis.
bioRxiv · Nov 2, 2026 · PreprintBioengineering