Jonathon Pines

This paper develops a stable RPE1 cell line with inducible and dose-dependent transgene expression using CRISPR/Cas9 and FRT/Tet-On systems, validated through multi-omics analysis.

This study shows that rapid degradation of CyclinA ensures proper chromosome congression and genomic stability by preventing aneuploidy and malformed nuclei.

This study identifies how the BTRR complex assembles on ultrafine DNA bridges through SLiM recognition by RMI1-RMI2, revealing spatiotemporal regulation during DNA repair and segregation.

The paper maps the ordered molecular events of SAC silencing at single kinetochores using MAD2 levels, revealing sequential unbinding, structural reorganization, and stabilization processes.

This study presents a proteomic and phosphoproteomic analysis of the human cell cycle in non-transformed cells, revealing new insights into cell cycle-dependent protein and phosphorylation oscillations.

The paper shows that spatial regulation of the APC/C at mitotic chromosomes enables rapid Cyclin B1 degradation, ensuring proper cell division and genomic stability.