Annalisa Roberti

This paper develops a stable RPE1 cell line with inducible and dose-dependent transgene expression using CRISPR/Cas9 and FRT/Tet-On systems, validated through multi-omics analysis.

This study shows that rapid degradation of CyclinA ensures proper chromosome congression and genomic stability by preventing aneuploidy and malformed nuclei.

The paper shows that environmental enrichment modulates age-related changes in the 3D chromatin interactome of mouse hippocampal neurons, inducing maturation in young mice and partial rejuvenation in old mice.

The paper shows that spatial regulation of the APC/C at mitotic chromosomes enables rapid Cyclin B1 degradation, ensuring proper cell division and genomic stability.