Dynamics of TFIIH and Spt4/5 during the transition from transcription initiation to elongation
James R. Portman, Kai Torrens, Gabriela G. Giordano, Jongcheol Jeon, Johnson Chung, Larry J. Friedman, Jeff Gelles, Stephen Buratowski
The paper uses single-molecule fluorescence microscopy to show that TFIIH dissociates from RNA polymerase II before Spt4/5 binds, indicating Spt4/5 does not actively displace TFIIH.
Proceedings of the National Academy of Sciences · Oct 28, 2026Peer-reviewedMolecular Biology