High-resolution fleezers reveal duplex opening and stepwise assembly by an oligomer of the DEAD-box helicase Ded1p
E. M. Patrick, R. Yadav, K. Senanayake, K. Cotter, A. A. Putnam, E. Jankowsky, M. J. Comstock
This study uses high-resolution optical tweezers to show Ded1p oligomers unwind RNA duplexes in 4-6 bp steps through dynamic protomer assembly and ATP hydrolysis.
bioRxiv · Feb 29, 2024Biophysics