Functional tagging of endogenous proteins and rapid selection of cell pools (Rapid generation of endogenously tagged piwi in ovarian somatic sheath cells.)
C. Marlin Andrews, P. Konstantinidou, P. Genzor, D. Stoyko, A. R. Elchert, L. Benner, S. Sobti, E. Y. Katz, Q. Meng, A. D. Haase
The paper presents a method to generate stable, endogenously tagged alleles in non-transformed cells, demonstrating its application in Drosophila ovarian somatic sheath cells for functional studies of Piwi.
bioRxiv · Dec 18, 2020Cell Biology