Short homology-directed repair using optimized Cas9 in the pathogen Cryptococcus neoformans enables rapid gene deletion and tagging
M. Y. Huang, M. B. Joshi, M. J. Boucher, S. Lee, L. C. Loza, E. A. Gaylord, T. L. Doering, H. D. Madhani
This paper optimizes Cas9 for Cryptococcus neoformans to enable efficient gene editing with short homology arms.
bioRxiv · Jun 30, 2021Microbiology