BldD-based bimolecular fluorescence complementation for in vivo detection of the second messenger cyclic di-GMP
M. Halte, M. E. Woermann, M. Bogisch, M. Erhardt, N. Tschowri
The paper introduces a c-di-GMP biosensor, CensYBL, that detects intracellular c-di-GMP levels in bacteria using bimolecular fluorescence complementation and validates its function in Escherichia coli and Salmonella enterica serovar Typhimurium.
bioRxiv · Aug 26, 2021Microbiology