M. Halte

Bacterial motility depends on a critical flagellum length and energy-optimised assembly

This study shows that bacterial flagellum assembly is optimized for energy efficiency and motility by balancing secretion rate and filament length.

FlhE functions as a chaperone to prevent formation of periplasmic flagella in Gram-negative bacteria

The paper identifies FlhE as a periplasmic chaperone that prevents the formation of periplasmic flagella in Salmonella, which disrupts cell wall synthesis and causes cell lysis.

Fluorescent tools for the standardized work in Gram-negative bacteria

This study evaluates 16 fluorescent proteins in Salmonella enterica to identify optimal tools for monitoring cellular processes, highlighting the benefits of degradation tags for improving signal stability and reducing cell-to-cell variability.

The F pilus serves as a conduit for the DNA during conjugation between physically distant bacteria

The study shows the F pilus acts as a DNA conduit during conjugation between distant bacteria, proposing a new model for DNA transfer.

Bacillus subtilis remains translationally active after CRISPRi-mediated replication initiation arrest

This study shows that Bacillus subtilis remains translationally active after CRISPRi-mediated replication initiation arrest, without DNA damage response or growth cessation.

A guide for membrane potential measurements in Gram-negative bacteria using voltage-sensitive dyes

This paper provides guidance on measuring membrane potential in Gram-negative bacteria using DiSC3(5) dyes, addressing challenges posed by the outer membrane and buffer conditions.

BldD-based bimolecular fluorescence complementation for in vivo detection of the second messenger cyclic di-GMP

The paper introduces a c-di-GMP biosensor, CensYBL, that detects intracellular c-di-GMP levels in bacteria using bimolecular fluorescence complementation and validates its function in Escherichia coli and Salmonella enterica serovar Typhimurium.