M. Brucale

Shared protocol to enrich and compare biochemical and biophysical properties of Extracellular Vesicles from human plasma and skeletal muscle biopsy

This paper presents a shared protocol to enrich and compare extracellular vesicles from human plasma and skeletal muscle biopsy, revealing distinct biochemical and biophysical properties between the two sources.

Compositional profiling of extracellular vesicles/lipoproteins mixtures by AFM nanomechanical imaging

This paper introduces an AFM-based method to distinguish and quantify lipoproteins and extracellular vesicles in mixed samples using nanomechanical imaging.

Stiffness of fluid and gel phase lipid nanovesicles: weighting the contributions of membrane bending modulus and luminal pressurization

This paper presents a model to quantify the contributions of membrane bending modulus and luminal pressure to the stiffness of lipid nanovesicles, validated through experiments and simulations.

Exploring the structure and mechanics of thin supported inverse bicontinuous cubic phase lipid films

This study creates and analyzes thin supported inverse bicontinuous cubic phase lipid films to understand their structure and mechanical properties.

Extracellular Vesicles Analysis in the COVID-19 Era: Insights on Serum Inactivation Protocols Towards Downstream Isolation and Analysis

This study compares heat and solvent-detergent inactivation protocols for serum samples intended for extracellular vesicles analysis, finding differences in EV recovery and contamination levels.

Gold Nanoparticles interacting with Synthetic Lipid Rafts: an AFM investigation

This study investigates how gold nanoparticles interact with synthetic lipid rafts using AFM, showing they preferentially adsorb at lipid phase boundaries.

AFM-based High-Throughput Nanomechanical Screening of Single Extracellular Vesicles

This paper introduces an AFM-based method to measure the mechanical stiffness and size of single extracellular vesicles by analyzing their deformation geometry.